In vivo chlorophyll fluorescence measurements
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Photo-physiological variability in phytoplankton chlorophyll fluorescence and assessment of chlorophyll concentration.
Photo-physiological variability of in vivo chlorophyll fluorescence (CF) per unit of chlorophyll concentration (CC) is analyzed using a biophysical model to improve the accuracy of CC assessments. Field measurements of CF and photosystem II (PSII) photochemical yield (PY) with the Advanced Laser Fluorometer (ALF) in the Delaware and Chesapeake Bays are analyzed vs. high-performance liquid chrom...
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The potential of measurements of chlorophyll fluorescence in vivo to detect cellular responses to salinity and degrees of salt stress in leaves was investigated for three crop plants. Sugar beet (Beta vulgaris L.) (salt tolerant), sunflower (Helianthus annuus L.) (moderately salt tolerant), and bean (Phaseolus Vulgaris L. cv Canadian Wonder) (salt intolerant) were grown in pots and watered with...
متن کاملThe fluorescence decay kinetics of in vivo chlorophyll measured using low intensity excitation.
We report fluorescence lifetimes for in vivo chlorophyll a using a time-correlated single-photon counting technique with tunable dye laser excitation. The fluorescence decay of dark-adapted chlorella is almost exponential with a lifetime of 490 ps, which is independent of excitation from 570 nm to 640 nm. Chloroplasts show a two-component decay of 410 ps and approximately 1.4 ns, the proportion...
متن کاملRelationship between the Absorption and Emission Spectra and the "Red Drop" in the Action Spectra of Fluorescence In Vivo.
The Stepanov equation, relating the intensity of emission, f(e)(v), at a given frequency, and that of absorption, k(v), at the same frequency, is applied, in its modified form (see equation 3 in text) to suspensions of Chlorella, Porphyridium, and Anacystis and to chlorophyll solutions. This application can reveal whether the yield of fluorescence, Phi(v), is constant, or changes with frequency...
متن کاملAnalysis of variable chlorophyll fluorescence in microphytobenthos assemblages: implications of the use of depth-integrated measurements
Measurements of in vivo chlorophyll a fluorescence made non-invasively on microphytobenthos samples represent the depth integration of the fluorescence emitted at different depths within the sediment. The effects of depth integration on fluorescence parameters and implications for the calculation and interpretation of indices used in quenching analysis were studied using a simulation model base...
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